The Adsorption and Desorption Capabilities of an Aluminum Silicate Analysis for Endotoxins
Julie L Brunkhorst, Amy Pope, Heather Henderson, Ronald Niemeijer
Trilogy Analytical Laboratory, Washington, MO 63090
Aluminum silicates are commonly used as additives in animal feeds to minimize the adsorption of mycotoxins and other toxic compounds by the animal. An evaluation was conducted to determine if Endotoxins could be analyzed for adsorption and desorption capabilities in an aluminum silicate. An Endotoxin solution was prepared in a buffered solution and then added to a recommended dosage of an aluminum silicate. The buffered solutions were then analyzed for adsorption and desorption using pH 5 for adsorption and pH 6.5 for desorption. Results proved that the analysis of Endotoxins could be performed with an aluminum silicate yielding 98.8% efficiency.
All solutions used in this analysis were made from LAL water and all glassware and test tubes were Endotoxin free.
Solutions made were:• 1N Hydrochloric Acid
• 1N Sodium Hydroxide
• pH 5 Buffer
• pH 6.5 Buffer
• Endotoxin Standard 40 EU/ml in pH 5 Buffer
All solutions were then tested for Endotoxins to determine background or concentration.
An aluminum silicate was weighed to represent 0.5 kg/ton and also 1kg/ton.
The 40 EU/ml Endotoxin in pH 5 buffer standard was added to the weighed aluminum silicates.
The samples were then incubated at 37°C for 3 hours while rotating.
Samples were then centrifuged and decanted into clean test tubes (any unbound Endotoxin will be in solution).
For desorption, pH 6.5 Buffer was added to the test tubes containing the bound Endotoxin.
The samples were then incubated at 37°C for an additional 3 hours while rotating.
Samples were then centrifuged and once again decanted into clean test tubes.
Adsorption and Desorption blanks, samples and standards were tested by a quantitative LAL FDA licensed cartridge system.
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